ZFIN Image: Zhang et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Tg(mylpfa:H2B-GFP) expression in jam3b^sa37 mutants. A: Tg(mylpfa:H2B-GFP) chevron-shaped expression pattern in the jam3b^sa37 mutant (indicated with an asterisk) allows easy identification from its non-mutant siblings under a fluorescence stereomicroscope. B: Fast-twitch muscle cells of 2 d.p.f. transgenic embryo displaying Tg(mylpfa:H2B-GFP) chevron-shaped expression pattern are mostly mononucleated, thus confirming its identity as a jam3b^sa37 mutant. Fast-twitch muscle cells are labeled with F310 antibody, with the nuclei and cell membranes stained with anti-GFP and anti-β-catenin respectively. Scale bar = 50 µm.

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 20(2), Zhang, W., Roy, S., The zebrafish fast myosin light chain mylpfa:H2B-GFP transgene is a useful tool for in vivo imaging of myocyte fusion in the vertebrate embryo, 106-10, Copyright (2016) with permission from Elsevier. Full text @ Gene Expr. Patterns