ZFIN Image: Felker et al., 2016, Fig. 1

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Figure Caption

Fig. 1

Experimental workflow to assess CreERT2 induction potency. Offspring of male ubi:creERT2 to female ubi:Switch crosses were induced with either trans-4-OHT or Endoxifen consistently at 50% epiboly. Imaging was performed at 4 dpf. Double-positive embryos were placed lateral (anterior to the left) and an image was taken of the whole embryo applying the same settings. Fluorescence was quantified using an automated Macro in ImageJ. Scale bar 500 µm. (C) To address a potential tissue bias due to possibly restricted drug penetration and recombination, we performed transverse vibratome sections of 4 dpf ubi:creERT2;ubi:Switch embryos induced under the same conditions as the embryos used for epi-fluorescence measurements (scale bar 50 µm). The depicted example images show switching in pectoral fin tissue after 4-OHT induction; merged: EGFP, mCherry and DAPI.

Acknowledgments

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