ZFIN Image: Koenig et al., 2016, Fig. 8

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Figure Caption

Fig. 8

vegfc and flt4 are dispensable for formation, but sufficient to induce ectopic growth of the SIV. (A and B) Based on in situ hybridization, vegfc expression is localized to the dorsal aorta, while flt4 shows expression in the progenitors of the intestinal vessels at 42 and 48 hpf (C and D). (E-H) In situ hybridization of vegfc^-/^-, flt4^-/-, and sibling embryos for fli1a. (E and F) vegfc^-/- embryos demonstrate normal SIV development at 52 hpf as compared to wild type and heterozygous siblings. Homozygous flt4^sa9798 (G and H) and flt4^hu4602 (I and J) embryos also have normal SIV development at 64 hpf and 52 hpf by in situ hybridization (G and H) and confocal microscopy of Tg(fli1a:GFP) embryos (I and J) as compared to wild-type and heterozygous siblings. (K and L) Heat shock induced overexpression of vegfc in hsp70: Vegfc embryos at 30 hpf results in hyperbranching and ectopic ventral sprouting of the SIV at 52 hpf as observed by confocal imaging of Tg(kdrl:mCherry). Large arrows indicate SIV. Anterior-left, dorsal-top.

Acknowledgments

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Reprinted from Developmental Biology, 411(1), Koenig, A.L., Baltrunaite, K., Bower, N.I., Rossi, A., Stainier, D.Y., Hogan, B.M., Sumanas, S., Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein, 115-27, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.