Fig. 3

Fig. 3

Mnx knockdown causes defects in tubular cilia arrangement and epithelial morphology. (A, B) Single plane confocal images showing tubule cilia stained by anti-actelytaled tubulin (red). Shown are lateral views of proximal tubules of control (A, A′) and mnx-morpholino injected Tg(cldnb:lynGFP) embryos (B, B) (C-F) Transverse TEM sections of proximal tubules in control (C, D) and mnx-morpholino embryos (E, F). Note disorganized microvilli structures after mnx gene knockdown (scale bars correspond to 5 µm (C, E) and 2 µm (D, F)). (G-J) Transverse sections of proximal tubules in control and mnx morphant embryos at 2dpf. Staining of baso-lateral surface directed alpha6F (G, H) and apically located Phalloidin (I, J) displayed similar expression patterns in control and MO-mnx. (K, L) Single plane confocal images of distal tubules stained for Phalloidin (red, ventral view). White arrows indicate displaced epithelial cells with unpolarized Phalloidin signals.