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Fig. 6
Mnx gene knockdown results in posteriorly expanded expression of irx1b. Flat-mount views of wild type and morpholino-injected embryos. Double-ISH analysis for either mnx2b or irx1b (blue) and pax2.1 (brown) shows localization within the nephric mesoderm at the 5som stage (A). Mesoderm expression of irx1b at the 8som stage is posteriorly expanded in mnx morphant embryos (C) compared to controls (B). Arrowheads point to posterior end of irx1b signals. Measurements of the distance from the posterior end of irx1b signals to the tail-bud are presented for single and double morpholino injected embryos (D).
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Reprinted from Developmental Biology, 411(1), Ott, E., Wendik, B., Srivastava, M., Pacho, F., Töchterle, S., Salvenmoser, W., Meyer, D., Pronephric tubule morphogenesis in zebrafish depends on Mnx mediated repression of irx1b within the intermediate mesoderm, 101-14, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.