ZFIN Image: Ott et al., 2016, Fig. 4

Image

Figure Caption

Fig. 4

Mnx1 negatively regulates irx1b expression. Microarray analysis identified differential expression of homeobox-containing genes in mnx1-VP16 induced animals compared to wild type controls. Genes attributed with the GO terms transcription regulation and homeobox that have increased expression in mnx1-VP16 animals are indicated (note that the robust mnx1 induction corresponds to the induced mnx1-VP16 transcripts). Shown are genes with >1.5 fold increase or reduction in expression after mnx1-VP16 activation (A). Of three identified irx family genes, irx1b showed the highest change in expression (labeled in green). Whole mount in situ hybridization for irx1b in wild type controls (B), mnx1 induced (C) and mnx1-VP16 induced (D) embryos, dorsal view. Negative regulation of irx1b through Mnx1 was further confirmed in qPCR analysis (E; p val <0.05 (*), <0.001 (***)).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 411(1), Ott, E., Wendik, B., Srivastava, M., Pacho, F., Töchterle, S., Salvenmoser, W., Meyer, D., Pronephric tubule morphogenesis in zebrafish depends on Mnx mediated repression of irx1b within the intermediate mesoderm, 101-14, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.