Fig. S1

Fig. S1

Growth of normal testicular fragments and testicular aggregates mixed with SSCs of testicular hyperplasia after transplantation. (A, B) The normal testicular fragment prior to the transplantation into rag1t²⁶⁶⁸³ mutant hosts (A), and the grafted testis fragment (B, enclosed by a dotted line) uncovered by peeling of host’s skin after 2 months of transplantation. (C, D) The aggregate of the inbred IM line that was mixed with spermatogonia of normal vas::egfp testes after 3 months of transplantion in the IM line. Arrows indicate GFP-positive cells (D). (E, F) The aggregate of the inbred IM line that was mixed with cultured SSCs of vas::egfp hyperplasias after 3 months of transplantation in the IM line. The SSCs were cultured for 4 weeks in the previous culture condition (Kawasaki et al., 2012). Arrowheads indicate GFP-positive cells (F). Sperm obtained from this aggregate generated fertilized embryos. Scale bar, 1mm.