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Fig. 2
H3 HCs rewire selectively with new ultraviolet cones.
(a) H3 HCs (yellow) in age-matched control and ultraviolet regenerated (UV Reg.) retinae at 15 d.p.f. visualized by one-cell stage DNA plasmid injection in the background of Tg(sws1:nfsB-mCherry, sws2:GFP) retina. Only the pedicles of the cones are shown here. (b) EdU (green) does not label ultraviolet cones in control retina (red asterisks), whereas many ultraviolet cones (red asterisks) are EdU positive after a period of regeneration (UV Reg.). The cell bodies and axon pedicles of the ultraviolet cones are shown. In the UV Reg. retina, orthogonal view of the H3 HC reveal dendritic tips inserting into EdU-positive regenerated ultraviolet cones (arrowheads). (c) Circles indicate the location of dendritic tips contacting each cone type. Green-filled circles are EdU-positive cones. Dendritic tips not associated with either ultraviolet and blue cones are assigned as undefined. (d-g). Quantitation of population data. (e) The dendritic field is defined as a polygon connecting the outermost dendritic tips. (g) Distance from the centre of mass of the cell soma to the farthest dendritic tip is quantified here. For all cells measured, the farthest contact was with an ultraviolet cone. For all plots, circles indicate values from individual cells. Error bars are s.e.m. NS, not significant; (d) P=0.42, (f) P=0.35, (g) P=0.12. P values are from Mann–Whitney rank-sum test. UV, ultraviolet.