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Fig. 1
Atrogin-1 is conserved between humans, mice, and zebrafish and zebrafish Atrogin-1 is expressed in the embryonic skeletal muscle and heart. (A) Protein alignment of Danio rerio (dr), Mus musculus (mm) and Homo sapiens (hs) Atrogin-1, identical amino acids are shaded in black, similar amino acids in grey. Conserved F-box domain is depicted in red; (B,B′) Immunofluorescent staining against Atrogin-1 on paraffin sections of zebrafish skeletal muscle at 72 hpf, nuclei are counterstained with DAPI, scale bar = 25 µm; (C-C′′′) Immunofluorescent staining against Atrogin-1 (green) and β-Catenin (red) on dissected zebrafish hearts at 72 hpf, nuclei are counterstained with DAPI, scale bar = 10 µm.