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Fig. 6
PT enhancer with hes6 3′UTR restricted the EGFP mRNA expression to the PSM. (A) EGFP reporter genes were used to generate the transgenic fish lines, Tg(hes6:EGFP-hes6 3′UTR). To generate the reporter gene used in Tg(hes6:EGFP-hes6 3′UTR), a DNA fragment corresponding to SV40pA in the reporter (shown in Fig. 2A) was replaced with hes6 3′UTR (474 bp). (B–O) Comparisons of EGFP mRNA distributions between Tg(hes6:EGFP-SV40pA) and Tg(hes6:EGFP-hes6 3′UTR) embryos. Scale bar, 200 µm.
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Reprinted from Developmental Biology, 409(2), Kawamura, A., Ovara, H., Ooka, Y., Kinoshita, H., Hoshikawa, M., Nakajo, K., Yokota, D., Fujino, Y., Higashijima, S.I., Takada, S., Yamasu, K., Posterior-anterior gradient of zebrafish hes6 expression in the presomitic mesoderm is established by the combinatorial functions of the downstream enhancer and 3'UTR, 543-54, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.