ZFIN Image: Miyagi et al., 2016, Fig. 11

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Figure Caption

Fig. 11

Effect of cardiac gata knockdown on promoter activation in skeletal muscle. (A and B) The 133-bp promoter construct with the GATA #1 mutation was co-injected into fertilized one-cell eggs along with control MOs (A) or gata4, gata5 and gata6-specific MOs (B). EGFP signals in the embryos were analyzed at 2 dpf. The arrow and arrowheads indicate EGFP signals in the heart and trunk muscle, respectively. The asterisks indicate yolk sac autofluorescence. Bars, 0.2 mm. (C) The bar graph represents the percentages of the embryos with EGFP expression in the trunk muscle. Data are expressed as the mean ± SEM of three independent experiments. No significant difference (ns) from the value of control-MO-injected embryos was found using Student′s t-test.

Acknowledgments

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Reprinted from Gene, 577(2), Miyagi, H., Nag, K., Sultana, N., Munakata, K., Hirose, S., Nakamura, N., Characterization of the zebrafish cx36.7 gene promoter: Its regulation of cardiac-specific expression and skeletal muscle-specific repression, 265-74, Copyright (2016) with permission from Elsevier. Full text @ Gene