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Fig. 3

ID
ZDB-IMAGE-160225-37
Source
Figures for Gokey et al., 2016
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Figure Caption

Fig. 3

Role of Kupffer′s vesicle size in LR patterning. A–D: Modulating Cftr activity was used to alter KV lumen size at 8 ss. The Cftr activating drug CFTact-09 increased KV size (B), whereas a loss-of-function mutation (C) or morpholino (MO) interference (D) reduced KV size relative to controls (A). Dashed circles represent approximate KV lumen boundaries. E–H: Possible outcomes of RNA in situ hybridization analysis of the LR patterning marker spaw in lateral plate mesoderm (arrowhead) at 18 ss are normal left-sided (E), bilateral (F), reversed right-sided (G), or absent (H) expression. Dashed lines indicate the embryonic midline. L = left; R = right. I–K: Quantification of KV area (I), cilia number (J), and cilia length (K) in embryos with altered Cftr function and controls. L: Analysis of spaw expression in Cftr modulated embryos. Error bars represent one standard deviation. N = number of embryos analyzed. * indicates P < 0.05.

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