|
Fig. 1
Differential behavior of localized determinants at the vegetal pole of the embryo.(A-D) Asymmetric movement of mRNAs for dorsal factors. Whole mount in situ hybridization shows that grip2a and wnt8a mRNA are localized at the base of the vegetal cortex and experience an off-center shift in control (DMSO-treated) embryos ((A) grip2a: 88%, n = 27 ; (C) wnt8a: 85%, n = 13). Treatment with nocodazole inhibits asymmetric movement ((B) grip2a: 84%, n = 19, (D) wnt8a: 85%, n = 13). (E-F) Symmetric localization of the germ line dazl mRNA. dazl mRNA is also localized to the base of the vegetal cortex in control embryos ((E) 90%, n = 11) and this distribution appears unaffected by nocodazole treatment ((F) 100%, n = 12). Embryos fixed at 40 mpf. Animal pole up, dorsal (when known) right. Arrowheads demark RNA localization domains.