IMAGE

Fig. 4

ID
ZDB-IMAGE-160205-100
Genes
Antibodies
Source
Figures for Khaliq et al., 2015
Image
Figure Caption

Fig. 4

id2a knockdown decreases hepatoblast proliferation and increases cell death in the developing liver. (A) Whole-mount immunostaining with anti-pH3 (red) anti-GFP(green) antibodies in Tg(prox1a:YFP) embryos. The total number of prox1a:YFP+ hepatic cells per liver is 316 ± 8.5 in controls and 156 ± 5.6 in id2a MO-injected embryos. (B) A graph showing the percentage of pH3+ cells among prox1a:YFP+ hepatic cells (n = 10). (C) EdU labeling (red), combined with anti-GFP immunostaining (green), in Tg(sox17:GFP) embryos reveals a significant reduction of proliferation in the liver of id2a MO-injected embryos at 40 hpf compared with controls. Dotted lines outline the liver. The total number of sox17:GFP+ cells per liver is 220 ± 16.6 in controls and 127 ± 12.7 in id2a MO-injected embryos. (D) A graph showing the percentage of EdU+ cells among GFP+ hepatoblasts (n = 10). (E) TUNEL labeling (red) combined with anti-Prox1 immunostaining (green) reveals apoptosis in the liver of id2a MO-injected embryos at 40 hpf, but not in controls. The total number of Prox1+ cells per liver is 276 ± 20.5 in controls and 140 ± 8.6 in id2a MO-injected embryos. (F) A graph showing the percentage of TUNEL+ cells among Prox1+ hepatoblasts (n = 10). *p < 0.05, **p < 0.005; error bars, ± s.e.m. Scale bars: 50 µm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 138 Pt 3, Khaliq, M., Choi, T.Y., So, J., Shin, D., Id2a is required for hepatic outgrowth during liver development in zebrafish, 399-414, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.