ZFIN Image: Powell et al., 2015, Fig. 5

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Fig. 5

cdonmRNA expression is regulated by Shh. cdon expression is slightly but significantly upregulated in the dorsal neural tube following Shh inhibition with cyclopamine as compared to control shown by ISH (A)–(D). Overexpression of Shh via heat shock results in loss of cdon expression. tg(hsp70l:shha-eGFP) embryos were heat shocked at 10 hpf and ISH was performed at 28 hpf for cdon expression. cdon is decreased within the dorsal trunk of Shh overexpressing embryos at 28 hpf (E–H; n=38 for heat shock; n=20 no heat shock). (I) qRT-PCR for cdon and patched 1/2 following Cyclopamine treatment show significant upregulation (p=0.0294) of cdon following Shh inhibition. Positive controls for Shh knockdown include patched1/2, a downstream readout of the canonical Shh signaling pathway, which is significantly reduced following cyclopamine treatment (p=0.0039). Gene expression was normalized to EF1a. Unpaired two tailed T-tests were performed in Graphpad (Prism).

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Reprinted from Developmental Biology, 407(2), Powell, D.R., Williams, J.S., Hernandez-Lagunas, L., Salcedo, E., O'Brien, J.H., Bruk Artinger, K., Cdon promotes neural crest migration by regulating N-cadherin localization, 289-99, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.