Fig. 4

Fig. 4

cdon is required cell-autonomously for NCC migration. Lateral views of live transplanted embryos, anterior to the left. Both transplanted donor cells sox10:eGFP and wildtype sox10:RFP host NCCs are visualized in the same embryo. (A)–(C) Presumptive NCCs from cdon morphant sox10:eGFP embryos were transplanted into the presumptive neural plate border region of 4 hpf wildtype sox10:RFP host embryos and imaged at 30 hpf. Donor cells from cdon morphants (green) were unable to migrate in wildtype host background (red), suggesting that cdon is required cell autonomously for NCC migration. (B) WT into WT and (E) WT into cdon MO transplants were performed as controls (showing only transplanted cells in green). Transplanted donor wildtype NCCs in either a wildtype or cdon MO background migrate normally. The sox10:RFP expression in these embryos was too weak to visualize in live embryos as shown. (F) Quantification of the fraction of cells transplanted as compared to the total number migrating in all conditions. For the calculations, at least 5 embryos were used for quantification: cdon MO into wildtype, 34 trunk NCCs transplanted in 11 embryos, only 5 cells migrated ventrally beyond the dorsal neural tube, indicating that only 16.5% were able to migrate in a wildtype environment as compared to 97% of cdon morphant cranial NCCs (155 cells transplanted in 13 embryos). For WT into WT transplants, 28 transplanted trunk cells in 6 embryos and 72 cranial cells in 5 embryos migrated ventrally. For WT into MO, 30 transplanted trunk cells in 6 embryos and 108 cranial cells in 5 embryos. WT, wildtype uninjected embryo; p<0.05. (G)–(I) Donor cells from cdon morphants (green) were able to migrate in wildtype host background (red) in the cranial region, suggesting that cdon is required cell autonomously for trunk NCC migration but not cranial NCC migration.