ZFIN Image: Barker et al., 2015, Fig. 6

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Figure Caption

Fig. 6

Most Gal4mpn354 Neurons Are Glutamatergic nsPVINs

(A) Single cell labeling of a Gal4mpn354 neuron using the BGUG method.

(B) Close-up and line drawing of the single Gal4mpn354 neuron shown in (A).

(C and D) Examples of co-localization of Gal4mpn354,UAS:GCaMP6s (green) expression and vglut2a:loxP-DsRed-loxP-GFP (magenta) or gad1b:loxP-DsRed-loxP-GFP expression (magenta).

(E) The majority of Gal4mpn354-labeled neurons are glutamatergic (VGlut2a; 76%). A minority are GABAergic (Gad1b; 22%). p = 4.8 × 10⁷; unpaired t test.

The scale bar in (A) represents 50 µm and in (B) represents 10 µm. Error bars indicate the SEM. p < 0.0005.

Acknowledgments

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