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Fig. 1 amotl2a is expressed in the pLLP and localizes at the cell apical side.
(A–I) cldnb:gfp embryos stained with an amotl2a antisense RNA probe and an anti-GFP antibody (E, G, I) at the indicated stages. Red arrows indicate the posterior lateral line primordium (pLLP). (D–I) Close-up views of the pLLP. (J–L′) Maximum intensity projection (MIP) of Z-stacks of the pLLP (J–J′) and a recently deposited neuromast (L–L′) in cldnb:gfp embryos injected with amotl2a-TdT mRNA. (K–K′) Close-up views of (J–J′). White arrows indicate rosette centers. Colors have been inverted. (M–X) 30 hpf cldnb:gfp embryos stained with an amotl2a in situ hybridization (ISH) probe and an anti-GFP antibody (O, R, U, X) in the indicated genetic background. The right column shows the primordium at higher magnification. In all figures, scale bars correspond to 200 µm for whole-embryo images and 20 µm in close up views of the pLLP. In all figures, n is the total number of embryos/primordia analysed and N is the number of biological replicates (Figure 1—figure supplement 1, Figure 1—source data 1).