Fig. 8

Fig. 8

Injection of z-foxg1 mRNA in zebrafish embryos causes delayed differentiation of olfactory neurons. a. Micrographs of double-positive Trpc2::Venus; OMP::CFP zebrafish embryos injected with control irrelevant mRNA (left) or with z-foxg1 mRNA (right), taken at 72 hpf. Arrows indicate the normal axonal pathway in the control embryos, asterisks indicate the regions of reduced fluorescence intensity, dotted lines outline the placode and the main axonal bundle. b. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control (irrelevant) mRNA (open bars) or with z-foxg1 mRNA (grey bars). A significant loss of both YFP and CFP + embryos is detected. Asterisks indicate p < 0.005. c. Whole-mount bright field micrographs of injected embryo, showing slightly altered embryonic morphology and nearly normal growth rate. d. (left) Quantification of endogenous z-OMPa, z-Trcp2, z-ngn1 and z-S100β mRNAs in embryos injected with z-foxg1 mRNA, by qPCR. Values are normalized against the control injection, set = 1. The over-expression of foxg1 causes a significant decrease of these mRNAs, with the exception of z-S100β. (right) Quantifications of z-hoxA7a and z-hoxA10b mRNAs in WT embryos injected with anti-z-foxg1 mRNA, relative to control injected embryos, used as in Fig. 5. Quantification of z-foxg1 mRNA was used to verify the injection.