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Fig. 2
The two compounds 12G9 and 36E9 inhibit pigmentation. The development of 12G9 and 36E9 treated embryos was slightly delayed. To analyze treated embryos at the same stage as control groups, these embryos were collected at 54 hpf. Lateral view, anterior to the left and dorsal to the top. A, C, E, and G show the live embryos; B, D, F, and H show in situ hybridization with dct probe. (A and B) DMSO control group. Treated embryos had stellate melanocytes with corresponding dct expression. (C and D) Embryos were treated with PTU. PTU blocked melanin synthesis and caused unpigmented embryos. However, the expression of dct showed that melanocytes were still present. (E and F) Embryos treated with 12G9. (G and H) Embryos treated with 36E9. Both 12G9 and 36E9 prevented pigmentation, and the number of dct positive cells in the trunk and RPE are much less than that of control group.