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Fig. 6
The proteasome inhibitor MG-132 blocks H2B-GFP degradation by Ab-SPOP.
293TetOn cells expressing H2B-GFP from a CMV promoter and FLAG-Ab-SPOP/RFP from a bi-directional TRE promoter were examined under epifluorescence 0 h (a) or 24 h (b) after doxycycline (1 µg/ml) and MG-132 (5 µg/ml) treatments. (C) H2B-GFP and FLAG-Ab-SPOP proteins levels were also investigated by immunoblotting with anti-GFP, anti-FLAG, and anti-actin antibodies.
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