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Fig. 3
Selective depletion of nuclear GFP-fusion proteins by Ab-SPOP.
293TetOn cells expressing Cdk4-GFP, Elf3-GFP, cMyc-GFP, or Pin1-GFP were transfected with vector expressing Ab-SPOPΔNLS or Abmutation-SPOPΔNLS from a bi-directional TRE promoter. ′–′ indicates untransfected cells. Expression of TagRFP and depletion of GFP were measured 8 hours after adding doxycycline (1 µg/ml) to media. Selective depletion of GFP in the nucleus, but not cytoplasm, was only observed in cells transfected with Ab-SPOP.
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