Fig. S3

Fig. S3

Depletion of GFP in nucleus, but not in cytoplasm, by Ab-­SPOP. 293TetOn cells expressing GFP were transfected with vectors expressing Ab-­SPOP_ΔNLS or Abmutation-­SPOP_ΔNLS a bi-­ directional TRE promoter. ′-′ indicates untransfected cells . Expression of TagRFP and depletion of GFP were measured after adding doxycycline (1 µg/ml) to media. TagRFP was first detected about 8 hours after induction of TRE promoters by doxycycline. Yellow arrowhead indicates cells in which GFP is depleted in the nucleus, but not in cytoplasm. Nuclear depletion of GFP started 3 hours after activation of TRE promoters and was only observed in cells transfected with Ab-­ SPOP.