ZFIN Image: Bednarek et al., 2015, Fig. S2

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Figure Caption

Fig. S2

Early activation of epicardial and endocardial genes is unaffected in the absence of telomerase.

(A) Representative images of aldh1a2 RNA and protein expression detected by in situ hybridization (ISH) and immunohistochemistry (IHC) in endocardium and epicardium at the indicated times post-injury in WT and tert^-/- zebrafish hearts. IHC on uninjured heart sections are shown for comparison. A total of n=4 hearts were analyzed per condition, with a minimum of 3 analyzed sections per heart. Asterisks mark the injured region. Boxed areas are shown at higher magnification. At, atrium; BA, bulbus arteriosus; dpi, days postinjury; V, ventricle. Scale bars: 100 µm for general views and 50 µm for zoomed views.

(B) RNA expression of endocardial genes (aldh1a2, il11a) and epicardial genes (wt1b, tbx18 and fibronectin) upregulated upon cardiac injury in WT and tert^-/- zebrafish hearts. Data are means ± SEM of values obtained from an RNA-seq experiment of 4 biological replicates, each replicate consisting of a pool of 3 hearts. Differences between WT and tert^-/- zebrafish hearts are non-significant in all cases (B-H adjusted p-value). CPM, counts per million; dpi, days postinjury; UI, uninjured.

Related to Figure 2.

Acknowledgments

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