ZFIN Image: Bednarek et al., 2015, Fig. S7

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Figure Caption

Fig. S7

Cardiac cells with accumulated DNA damage are less prone to proliferate.

(A) Immunofluorescence staining on a WT and a tert^-/- heart section with anti-BrdU (red) and anti-γH2AX (green). Nuclei are counterstained with DAPI (blue). (A1-A4) Boxed areas in the whole-mount heart views of WT (A1,A2) and tert^-/- (A3,A4) are shown at high magnification in the right panels to exemplify individual BrDU-positive cells. Scale bars: 100 µm (whole mount views), 10 µm (magnifications).

(B) Quantification of cells double positive for BrDU and γH2AX. Data are means ± SEM of 4 sections/heart from a total of 3 hearts per condition (p=0.106, unpaired Student’s t-test). ns, not significant.

Related to Figure 6.

Acknowledgments

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