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Fig. 3
Linear unmixing of EGFP and eYFP.
(a) Schematic drawing of a two days post fertilization zebrafish embryo imaged dorsally. (b) Integration of the reconstructed data set from a Tg(kdrl:EGFP,s1013t:Gal4,UAS:ChR2-eYFP) zebrafish along γ. Contributions from EGFP and eYFP could not be distinguished. (c) With linear unmixing, EGFP (cyan) and eYFP (red) were separated and (d) their spectra determined: measured spectrum (grey), EGFP (cyan), eYFP (red). Values from previous studies16 (dashed) are plotted for comparison. Maximum intensity projections of seven planes, z-spacing 10µm. Scale bar, 100µm.
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Full text @ Nat. Commun.