ZFIN Image: Mosimann et al., 2015, Fig. S6

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Figure Caption

Fig. S6 Impact of tbx5a and pitx2ab knockdown on SHF addition to the FHF. Scale bar = 10 µm. (a-i) Photoconversion-mediated lineage tracing of the SHF cardiomyocytes addition to the linear heart tube. Photoconversion of cardiomyocyte-specific Tg(myl7:nlsKikGR) was performed at 365 nm for 2 minutes at 27 hpf and the hearts imaged at 56 hpf; the total conversion to RFP was visually confirmed. a, d, g, shows the green channel, b, e, h, shows the red channel, merge is shown in c, f, i. All GFP-positive nuclei are the newly added cardiomyocytes after 27hpf, RFP-labeled nuclei represents all cardiomyocytes of the linear heart tube prior to photoconversion, yellow in merge (c, f, i). a-c, control hearts, d-f, hearts from tbx5a morphants show mild decrease in newly added cardiomyocytes, g-i, hearts from pitx2ab morphants display increase in cardiomyocyte recruitment.

Acknowledgments

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