IMAGE

Fig. 6

ID
ZDB-IMAGE-151029-21
Genes
Antibodies
Source
Figures for Chen et al., 2015
Image
Figure Caption

Fig. 6

lamb1a induction defines cell polarity and signaling competence in basal cells of the regeneration epidermis.

(A) Antibody co-staining for aPKC (red) and P63 (green; an epithelial maker for all basal and some suprabasal epithelial cells) in longitudinal sections of sde1/+ and sde1 fin regenerates at 2 dpa. (B) Antibody staining for Laminin in fin regenerates at 4 dpa after a temperature shift from 25°C to 33°C at 3 dpa, indicating induced mislocalization. Laminin, red; DAPI, blue. White dashed boxes indicate areas of enlarged view. (C) Antibody co-staining for aPKC (red) and P63 (green) in longitudinal sections of sde1/+ and sde1 fin regenerates at 4 dpa after a temperature shift from 25°C to 33°C at 3 dpa, indicating loss of basal cell polarity. iii: distal regenerated tissue (sde1/+); iv: distal regenerated tissue (sde1). White arrows indicate basal cell nuclei. (D) Antibody co-staining for phosphorylated Igf1r (red) and P63 (green) in longitudinal sections of 5 dpa sde1/+ and sde1 fin regenerates, after a temperature shift from 25°C to 33°C at 4 dpa. The basal localization of p-Igf1r is enriched in basal epithelial cells in sde1 mutants. (E) shha RNA expression is reduced in sde1 fin regenerates at 4 dpa after a temperature shift from 25°C to 33°C at 3 dpa. Scale bars, 50 µm. White dashed boxes indicate areas of enlarged view. Red arrows indicate plane of amputation.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.