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Fig. 5
Element B1B2-761bp is a true enhancer and not a cryptic promoter. Transient expression of EGFP placed under the control of B1B2-761bp DNA fragment in native (A,C,E,G,I,K) and inverted (B,D,F,H,J,L) orientation. DNA was injected into wild-type zebrafish embryos at 1-cell stage in absence of Tol2 transposase. Live embryos were anesthetized with tricaine and mounted in agarose before imaging on a Zeiss epifluorescence microscope. All images are single z-axis sections. Embryonic stages imaged were: 30 hpf (A,B), 52 hpf (D–F), 76 hpf (G–L). Scale bar: 50 µm in panels A and B; 20 µm in panels C–L.
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