IMAGE

Fig. S1

ID
ZDB-IMAGE-151001-10
Genes
Source
Figures for Yang et al., 2015
Image
Figure Caption

Fig. S1

VEGF and Notch regulate PLK2 expression in HUVECs and zebrafish vasculature. (A)RT-PCR and (B) Western analysis reveals that PLK2 mRNA and protein expression levels were elevated in HUVECs after VEGF treatment. (C) Quantitative measurements show that PLK2 mRNA (p=0.004) and protein level (p=0.015) in HUVECs is less after VEGF treatment. (D–G) Whole mount RNA in situ hybridization shows that (E, E′) VEGF pathway inhibition with SU5416 (n=29/33) treatment starting at 20 hpf resulted in decreased plk2b expression in the zebrafish vasculature at 32 hpf when compared to (D, D′) DMSO treatment (n=0/35), whereas (G, G′) Notch inhibition with dn-MAML overexpression (n=24/35) starting at 20 hpf resulted in increased plk2b expression in the zebrafish vasculature at 32 hpf when compared to the (F, F′) control siblings (n=0/35). D′–G′ images represent the boxed area in D–G. (H, I) Quantitative PCR confirms that plk2b expression level in sorted endothelial cells is decreased in (H) SU5416 treated fish (p=0.0178) and (I) increased in dn-MAML overexpression fish (p=0.0142). Mean±s.e.m. *p<0.05, **p<0.01 by Student′s t-test.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 404(2), Yang, H., Fang, L., Zhan, R., Hegarty, J.M., Ren, J., Hsiai, T.K., Gleeson, J.G., Miller, Y.I., Trejo, J., Chi, N.C., Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development, 49-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.