|
Fig. 3 Retinoblastoma invasion and metastasis in the zebrafish
(a) Time-course monitoring invasion and metastasis of DiI-labeled mouse SJmRBL-8 retinoblastoma cells (red color) in the head region of zebrafish embryos. Arrows indicate metastatic tumor cells in single cell or a cluster cells. Bar = 100 µm. (b) Quantification of percentage of zebrafish embryos with contralateral ocular SJmRBL-8 metastasis, percentage of zebrafish embryos with head metastasis, percentage of zebrafish embryos with trunk metastasis, and the average total number of metastatic tumor cells per zebrafish (n = 40 embryos/group). (c) Time-course monitoring invasion and metastasis of DiI-labeled human RB355 retinoblastoma cells (red color) in the head region of zebrafish embryos. Arrows indicate metastatic tumor cells in single cell or a cluster cells. Bar = 100 µm. (d) Quantification of percentage of zebrafish embryos with contralateral ocular RB355 metastasis, percentage of zebrafish embryos with head metastasis, percentage of zebrafish embryos with trunk metastasis, the average total number of metastatic tumor cells per zebrafish, and the distribution of metastatic SJmRBL-8 and RB355 tumor cells in head and trunk regions at day 4 after primary tumor implantation (n = 40 embryos/group).