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Fig. S7 Evidence that ubap1 is not the gene-trap target in line fcc143.
(A) RT-PCR analysis of ubap1 and actin expression in control and ubap1 morphants co-injected with p53 morpholino. (B) Brightfield images of groups of control and ubap1 morphants. Ubap1 morphants display widespread developmental defects, unlike fcc143 GFP-high embryos (see Fig 4B). (C) WISH of ubap1 antisense and sense probes in 2 and 6 dpf embryos. Ubap1 expression, shown by the antisense probe, was not detected in the caudal hematopoietic tissue or thymus in contrast to the GFP pattern in fcc143 carriers (see Fig 2).
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