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Fig. S2

ID
ZDB-IMAGE-150921-19
Source
Figures for Wei et al., 2015
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Figure Caption

Fig. S2

The HR factors are specifically recruited at transcriptionally active damage sites in both U2OS TRE and U2OS 263 cell lines. (A) Recruitment of LIG4, BRCA1, CtIP, and BRCA2 at TA-KR and tetR-KR damage sites in U2OS TRE cells 10 min after light irradiation is shown. (B) U2OS TRE cells were cotransfected with GFP tagged RAD52 or RAD51C and tetR-cherry or TA-cherry; 24 h after transfection, cells were illuminated with fluorescent light for 10 min. There is no recruitment of these factors at sites of tetR-cherry or TA-cherry. (C) The recruitment of the HR factors in U2OS 2-6-3 cells. U2OS 263 cells were cotransfected with GFP tagged RAD52, or RAD51C and tetR-cherry/KR or TA-cherry/KR; 24 h after transfection, cells were illuminated with fluorescent light for 10 min. RAD51 was stained with antibody. The recruitment of these factors specifically at sites of TA-KR is shown. (D) Quantification of average fold increase of foci intensity (n = 10) of the NHEJ factor at sites of tetR-KR or TA-KR at the indicated recovery time after damage. Error bars indicate the SEM of three independent experiments.

Acknowledgments
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