ZFIN Image: Gao et al., 2015, Fig. S2

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Figure Caption

Fig. S2 The primitive hematopoiesis is undisturbed in mutant^cas003.

(A-L) WISH results showing normal expression of scl, gata1, pu.1, lyz, l-plastin and mpx at 22hpf in mutant^cas003 embryos comparing to siblings. Total numbers of embryos with scl or gata1 stain are 56 and 76, respectively. The penetrance of the indicated phenotype is shown in the bottom left of each panel in C-L. Blue arrows indicate the myeloid cells in the yolk sac; black arrows indicate the granulocytes in the posterior blood island (PBI). (M)Quantitative analysis of pu.1⁺, lyz⁺, l-plastin⁺ and mpx⁺ cell numbers showing no significant difference between sibling and mutant^cas003 embryos at 22hpf. Error bars represent SEM. ns represents no significance.

Acknowledgments

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