IMAGE

Fig. 4

ID
ZDB-IMAGE-150915-47
Genes
Source
Figures for Gao et al., 2015
Image
Figure Caption

Fig. 4 The defect of definitive hematopoiesis is due to the increased apoptosis in the HSPCs of topbp1cas003 mutants.

(A-D′) The c-myb in situ hybridization and phospho-histone H3 (pH3) immunostaining at 3.5dpf in sibling and topbp1cas003 mutant embryos, indicating no significant difference in the number of pH3+ HSPCs (pH3 and c-myb double positive cells, white arrows) between wild-type sibling and topbp1cas003 mutant embryos. The images show the stain in the enlarged CHT region. Scale bars represent 25µm. (E-H′) Double immunostaining of c-myb: EGFP and BrdU at 3.5dpf in the sibling and topbp1cas003 mutant embryos. The number of BrdU+ HSPCs (BrdU and EGFP double positive cells, white arrows) in topbp1cas003 mutant embryos is comparable to that in wild-type siblings. Scale bars represent 25µm. (I-P′) Double immunostaining of c-myb: EGFP and TUNEL at 3.5dpf (I-L′) and 4dpf (M-P′) in the sibling and topbp1cas003 mutant embryos. The apoptotic HSPCs (TUNEL and EGFP double positive cells, white arrows) are increased in the topbp1cas003 mutants comparing to siblings, which is more profound at 4dpf. Scale bars represent 25µm. (Q) Quantification of the number of the pH3+ HSPCs in sibling and topbp1cas003 mutant embryos at 3.5dpf. (R) Statistics result of the percentage of BrdU+ HSPCs at 3.5dpf. (Q) and (R) indicate no significant difference in the number of proliferating HSPCs between topbp1cas003 mutant embryos and siblings at 3.5dpf. (S) Quantitative analysis of apoptotic HSPCs in the CHT region in sibling and topbp1cas003 mutant embryos at 3.5dpf and 4dpf, showing the increased apoptotic HSPCs in the topbp1cas003 mutant embryos. For the Quantitative analysis, at least 6 embryos were analysis for each experimental group. Error bars represent SEM. *, p<0.05; ***, p<0.001.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.