IMAGE

Fig. 5

ID
ZDB-IMAGE-150811-17
Genes
Source
Figures for Xue et al., 2015
Image
Figure Caption

Fig. 5

klf6a knockdown induces a blockage of erythroid cell differentiation and maturation. (A) WISH results showed that gata1 expression was comparable between controls and klf6a morphants from 24 hpf. The expression of be1-globin and be3-globin was decreased in klf6a morphants, compared with controls at 36 hpf and 2 dpf. (B) The number of o-dianisidine-positive cells (marked by arrowheads) decreased dramatically in klf6a morphants between 40 hpf and 2 dpf. (C) The real time PCR results displayed that ae1-globin and be1-globin were downregulated in klf6a morphants at 36 hpf and 2 dpf (mean±SD, *P <0.05, **P <0.01, n=3). (D) Giemsa staining of erythroid cells from the blood of controls and klf6a morphants at 2.5 dpf. The size and nucleo-cytoplasmic ratio of red blood cells in klf6a morphants were noticeably larger than those of controls. The arrowhead marks the delayed erythroid progenitor. (E) Quantification of the nucleo-cytoplasmic ratio between controls and klf6a morphants (mean±SD, NNP <0.01, n=19).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 403(2), Xue, Y., Gao, S., Liu, F., Genome-wide Analysis of the Zebrafish Klf Family Identifies Two Genes important for Erythroid Maturation, 115-27, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.