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Fig. S3 Treatment with AG1478 does not induce apoptosis in the optic tectum.
A. Immunohistochemical staining of embryos treated with AG1478 and the control DMSO with anti-activated Caspase-3 antibody at 38 hpf. Arrow, Caspase-3-positive puncta; dotted circle, optic tectum (OT). B. Quantification of the number of Caspase-3-positive puncta in the OT for the experiment shown in A (mean ± s.e.m.; DMSO, 2.4 ± 0.93, n = 5, AG1478, 3.0 ± 1.00, n = 5, p = 0.67, unpaired t test).
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