ZFIN Image: Sato et al., 2015, Fig. S7

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Figure Caption

Fig. S7 MO^nrg1IIatg specifically suppresses ectopic expression of NRG1-II.

A. A schematic structure of an expression plasmid CMV:nrg1II-EGFP (top), a part of the nucleotide and amino acid sequences encoding 5′ untranslated and coding regions in the first exon of nrg1-II (middle), and the target sequence of MO^nrg1IIatg (bottom, red arrow). B. Representative 74-hpf embryos co-injected with CMV:nrg1II-EGFP expression plasmid and MO^nrg1IIatg or the control MO^nrg1IIatg5m shown in a lateral view. Green fluorescence in yolk of MO^nrg1IIatg-injected embryos is autoflurescence (arrowhead). Scale bar, 500 µm. C. Quantification of ratios of GFP-positive embryos co-injected with CMV:nrg1II-EGFP and MO^nrg1IIatg or the control MO^nrg1IIatg5m at 25 hpf. No GFP-positive embryos were detected for MO^nrg1IIatg-injected embryos under a fluorescent dissection microscopy. (mean ± s.e.m.; nrg1IIatg5m, 0.56 ± 0.08, n = 183 embryos by 4 injections, nrg1IIatg, 0.00 ± 0.00, n = 98 embryos by 4 injections).

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