Fig. S5

Fig. S5 Defective generation of GFP-expressing neurons in the optic tectum by injection of antisense MOs against nrg1.

A. Knockdown of all isoforms of NRG1 by injection of MO^nrg1EGF. Representative embryos injected with MO^nrg1EGF (right) or the control MO^ctrl (left) shown in a lateral view at 48 hpf. Dotted circle, optic tectum (OT). Scale bar, 100 µm. B. Quantification of pou4fl-hsp70l:GFP intensity in the OT for the experiment shown in A (mean ± s.e.m.; ****P < 0.0001, unpaired t test; n = 6–7). C. Impaired neurogenesis in a representative MO^nrg1IIE1-injected Tg(pou4f1-hsp70l:GFP) embryo (right) compared to the control MO^nrg1IIE15m (5 nucleotides-mismatched control)-injected embryo (left) at 50 hpf. Scale bar, 100 µm. D. Quantification of pou4f1-hsp70l:GFP intensity in the OT for the experiment shown in C (mean ± s.e.m.; ****P < 0.0001; n = 8 per group). E. Knockdown of membrane-bound isoforms of NRG1 by injection of MO^nrg1TM. Representative embryos injected with MO^nrg1TM (right) or the control, MO^nrg1TM5m (left) shown in a lateral view at 53 hpf. Dotted circle, OT. Scale bar, 100 µm. F. Quantification of pou4f1-hsp70l:GFP intensity in the OT for the experiment shown in E (mean ± s.e.m.; ****P < 0.0001, unpaired t test; n = 6 per group).