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Fig. S4 Generation of GFP-expressing neurons in the optic tectum is partially recovered after removal of AG1478.
A. A timeline of experiments of AG1478 treatment. Embryos were soaked into 25 µM AG1478 solution or the control DMSO from 25 to 50 hpf. Then, they were washed and grown in a fresh medium. Embryos at 72 hpf were collected for analyses. B. Decreased pou4f1-hsp70l:GFP-positive neurons in the optic tectum of AG1478-treated embryos at 50 hpf were partially recovered at 72 hpf compared to the control DMSO in Tg(pou4f1-hsp70l:GFP).
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