ZFIN ID: ZDB-IMAGE-150609-7
Figures for Love et al., 2015

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Fig. 1

Maternal rest is required for proper FBMN migration. (A) PCR genotyping of mating crosses to produce rest zygotic and maternal–zygotic (MZ) mutant embryos. The restsbu29 allele introduces a 7 bp deletion resulting in a smaller PCR product (red arrowhead). Homozygous mutant females (red box) produced eggs lacking maternal rest transcript. (B–F) Average intensity projection confocal images (dorsal views) of Tg(islet1:GFP); restsbu29 mutant embryos at 48 hpf, immunolabeled with EphA4a to allow visualization of r5 (red). Scale bar=20 µm. Decreasing levels of rest from B to F correlate with increasing disruption in FBMN migration. (B′–F′) Pie charts represent the average number of FBMNs counted in r4 (black), r5 (checkered), and r6 (white) as a percentage of total FBMNs in rest mutant embryos. The total number of FBMNs was not significantly different in embryos of different genotypes. (G) Statistical analysis of FBMN migration in restsbu29 mutants. The percentages of FBMNs that fully migrate to r6 (white) were compared using unpaired t test. Parentheses indicate number of embryos analyzed per group. Mean±SEM; *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001; n.s.=not significant. (H and I) Tg(islet1:GFP) embryos co-injected with both splice- and translation-blocking morpholinos targeted against Rest recapitulate the MZrestsbu29/sbu29 mutant phenotype at 48 hpf. Scale bar=20 µm. (J and K) MZrestsbu29/sbu29 FBMNs fail to reach r6 at 72 hpf, indicating that the mutant phenotype is not merely a consequence of developmental delay.

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