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Fig. 4
MiRNA 451 is required for maintenance of erythroid cells in zebrafish. (A) WISH analysis for expression of erythroid markers in wild-type and miR 451 morphant embryos at H24 hpf. At this stage, committed erythroid cells are relatively immature and blood circulation is not yet established. The expression of erythroid markers in morphant embryos is unchanged, indicating normal specification of primitive erythropoiesis. The morphant embryos showed strongly reduced expression of miR 451 (SI Fig. 10). (B) WISH analysis for expression of βe1-globin in wild-type and miR 451 morphant embryos at H48 hpf. At this stage, mature erythroid cells have entered the circulation. The ducts of Cuvier on the yolk (square bracket, 1) and the cardinal vein (arrow, 2) are indicated, and higher-magnification views are shown in Lower. (C) o-dianisidine staining for hemoglobin in morphant embryos at 48 hpf.