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Fig. S4 Obesity in 6.5 months old HF-LD fish is accompanied with differential and region-specific hyperplasia and hypertrophy of subcutaneous adipocytes, while keratinocytes are largely unaffected.
A: Schematic overview of the different dorsoventral levels (1–5) at which longitudinal sections were used to analyze subcutaneous adipocytes; B: overview of an H&E stained section of a male HF-LD fish at level 2; boxes show locations of areas shown in Fig. 3 A-H. C,D: Numbers of subcutaneous adipocytes per level (C) and sizes (D) of subcutaneous adipocytes of male and female LF-HD and HF-LD fish in each of the 5 analyzed levels, n = 8 (2 fish per condition, 2 sections per level and 2 sides per section); E,F: Thickness of the skin (E) and sizes of keratinocytes (F) of male and female LF-HD and HF-LD fish, determined at the level of the most posterior scale for each level, means of all levels are shown; columns with same superscript letter are not significantly different (p>0.05) according to ANOVA followed by the Least Significant Difference (Bonferroni’s) test, n = 20 (for thickness, 2 fish per condition, 5 measurements per side of the analysed section) or n = 40 (for size, 2 fish per condition, 10 measured keratinocytes per side of the analysed section). Similar results were obtained in second, independent experiments.