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Fig. 4

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Figures for George et al., 2015
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Fig. 4

nkx2.5 is necessary to maintain ventricular identity in late-differentiating cardiomyocytes derived from the SHF. MF20/S46 immunofluorescence distinguishes ventricular myocardium (white) from atrial myocardium (green) in non-transgenic (A-F) and Tg(hsp70l:nkx2.5-EGFP) (G-L) embryos. Confocal projections of wild-type (A-C, G-I) and nkx2.5-/- (D–F, J–L) hearts depict cardiomyocyte nuclei with Tg(-5.1myl7:nDsRed2) (red). Ventral views, arterial pole to the top, at 55 hpf. (B,C,E,F,H,I,K,L) White dots outline the MF20+ cardiomyocyte borders in the OFTs of hearts in (A,D,G,J), respectively. White arrows indicate ectopic S46+ cells; “A” denotes atrium. All embryos were heat-shocked at 11 somites. (A–C) In wild-type non-transgenic hearts, the late-differentiating cardiomyocyte population exhibits MF20, but not DsRed, fluorescence due to the delay in expression of Tg(-5.1myl7:nDsRed2) at the arterial pole. (D–F) Similarly, in non-transgenic nkx2.5-/- hearts, cardiomyocytes expressing MF20, but not DsRed, are present at the arterial pole. A few ectopic S46+ cardiomyocytes are also visualized in this region (DsRed nuclei). (G–I) In transgenic wild-type hearts, MF20, but not DsRed, fluorescence at the arterial pole designates the delayed differentiation of these SHF-derived cardiomyocytes. (J–L) Despite the rescue of the cardiac chamber morphology and identity, excess S46+ cardiomyocytes are visualized at the arterial pole of transgenic nkx2.5-/- hearts where the late-differentiating population accretes (DsRed nuclei).

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Reprinted from Developmental Biology, 400(1), George, V., Colombo, S., Targoff, K.L., An early requirement for nkx2.5 Ensures first and Second heart field ventricular identity and cardiac function into adulthood, 10-22, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.