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Fig. 2
CaM expression in zebrafish does not affect embryo development or heart morphology. (A) Representative images of 72 hpf embryos from eggs injected with CaM variant mRNA. Embryos overexpressing CaM WT, N97S or N53I display normal morphology and development. (B) Z-stack images of 72 hpf embryonic hearts that were microdissected and immunostained for slow muscle fibres (F59). (C) Developmental rates measured by counting somites at 22 hpf. No significant difference between control, CaM WT, N97S and N53I injected embryos was observed (P = 0.85).
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