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Fig. S3 F-actin in microridges is highly dynamic.
Photoconversion of actin-mEos was performed on 3 dpf larvae by focusing on a small region of an epithelial cell containing microridges (red circle) and acquiring time-lapse images before (labeled with blue frame) and after photoconversion. Upon photoconversion, mEos-actin is irreversibly converted from exhibiting green to red fluorescent emission. Data are representative of 15 individual cells. For details of photoconversion refer to Materials and Methods. Single plane of confocal image of red channel, green channel and overlay of the two are shown. Scale bar, 10 µm. See S6 Movie.