ZFIN Image: Semmelhack et al., 2014, Fig. 6

Image

Figure Caption

Fig. 6

Ablation of AF7 markedly reduces prey capture behavior.

(A) Intact Ath5:Gal4, UAS:Dendra larva at 8dpf. (B) Ath5:Gal4, UAS:Dendra larva after ablation of AF7 neuropil. (C) Pre-ablation response to paramecia in one example larva. Behavior was recorded for 5 min and the videos were edited to display all swim bouts. Each bout was classified by the same SVM as in Figure 4C. (D) The same larva after 2P laser ablation of AF7. (E) Total duration of prey capture bouts during the five 1 min trials before and after AF7 ablations. n = 14 larvae. p = 2.59 × 10⁴, Wilcoxon rank sum test. (F) Prey capture time before and after AF9 ablations. n = 15 larvae. (G) Duration of prey capture bouts after ablation as a percent of initial prey capture time. p = 2.68 × 10⁴, Wilcoxon rank sum test. (H) Optomotor response to moving gratings after AF7 and AF9 ablation. AF7ablation n = 7 larvae, AF9 ablation n = 9 larvae. p = 5.2 × 10³, Wilcoxon rank sum test. Error bars = ±SEM. See Figure 6—figure supplement 1 for AF7 and SO axon anatomy and periventricular neuron activity before and after ablation.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife