IMAGE

Fig. 6

ID
ZDB-IMAGE-150318-30
Source
Figures for Lu et al., 2014
Image
Figure Caption

Fig. 6

Zebrafish Abcc4 has functions in transport of MCB out of LLC-PK1 cells.

(A) Western blot analysis of Flag-tagged Abcc4 or Abcc4-G1188D in stably transfected LLC-PK1 cells and the control cells (CTRL) transfected with empty vectors. The β-actin was used as a loading control. (B) Green signals under the confocal microscope indicate that foreign Abcc4 or Abcc4-G1188D molecules are localized on plasma membrane of LLC-PK1 cells. (C) Fluorescence intensities in LLC-PK1 cells expressing Abcc4 or Abcc4-G1188D and the control (CTRL) after treatment with MCB for 30 min at indicated concentrations. (D) Fluorescence intensities in LLC-PK1 cells expressing Abcc4 or Abcc4-G1188D and the control (CTRL) after treatment with 25 µM MCB for indicated time periods. (E) Fluorescence intensities in Abcc4-expressing LLC-PK1 cells after treatment with MK571 for indicated time periods. Cells were incubated in medium containing 25 µM MCB and different concentrations of MK571. Data are expressed as means ± standard deviations (n = 3). Significant differences are indicated by *p<0.05 and **p<0.01.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One