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Fig. 1
Transcriptional expression of zebrafish abcc4 gene.
(A) Spatiotemporal expression of abcc4 gene in developing embryos at indicated stages was detected with whole-mount in situ hybridization (WISH). (a) 1 hpf, lateral view; (b) 6 hpf, lateral view with dorsal to the right; (c) 12 hpf, lateral view with anterior to the top and dorsal to the right; (d) 24 hpf, lateral view with anterior to the left; (e, g, h and j) 48, 72, 96 and 120 hpf, lateral views with dorsal to the right; (f, i and k) 48, 96 and 120 hpf, dorsal views with anterior to the top. ey, eyes; le, lens; pa, pancreas; gi, gills; in, intestine; bl, swim bladder. (B) Transcriptional analysis of zebrafish abcc4 gene in developing embryos. Sixty embryos at 1-hpf stage or thirty embryos at 6- to 120-hpf stage were pooled for total RNA extraction and subjected to qPCR analysis. (C) Tissue-specific expression of abcc4 gene in adult zebrafish. Values are given as mean ± standard deviation, n = 3.
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