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Fig. S1 sox21a morpholino activity assay. A) 1 Cell stage zebrafish embryos were injected with mRNA encoding for sox21a fused to a N terminal Myc tag, which lacks the target site for the sox21a morpholino, thereby being insensible to it. Injections were performed with this mRNA alone (left) or mixed with the sox21a morpholino (right). In both cases the nuclear Myc tag is detected by immunostaining (29). B) A similar approach was performed using a mRNA encoding for sox21a fused to a C terminal Myc tag, which is sensible to the sox21a morpholino. In this case, the Myc tag is only detected when the mRNA is injected alone (left) while the co-injection with the sox21a morpholino efficiently targets the mRNA and blocks its translation.